Platform

Our synthetic libraries have a great diversity and they have their own backbone structure in which only (parts of) the CDR regions are made variable. The fixed and variable DNA fragments of these nanobodies have been cloned into plasmids that have subsequently been incorporated into suitable E. coli strains. Using M13 phages, we can then express the nanobody in question and then screen and select it in various ways. The most ideal option is to ultimately screen for targets that are identical to the intended application of the nanobody.